The flours obtained from described treatments were extracted in a batch process using analytical grade hexane (J.T. Baker, Mallinckrodt Baker Inc., Phillipsburg, NJ) in 1:6 (w/v) flour to hexane ratio and at constant temperature of 35 C during 15 min of extraction time (Navarrete-Bolan˜os, Jime´nez-Islas, Rico-Martı´nez, Domı´nguez-Domı´nguez, & Regalado-Gonza´lez, 2001). When the extraction was concluded, the light phase (hexane + xanthophylls) must be separated of the heavy phase (solid). The light phase was concentrated to obtain the oleoresin extract that was weighed (digital analytical balance, Ohaus-explorer) to quantify the yield extraction and determine the xanthophylls concentration according to AOAC (1984) method 970.64. The heavy phase (solid) was desolventized, and the retained solvent volume was determined by difference of weights (Ohaus-explorer balance). Solvent-free solid was also analyzed to determine the xanthophylls concentration according to AOAC (1984) method 970.64.