เปลี่ยนแปลงระหว่างสีขาว และสีม่วง morphsConsidering the three orchid species, and considering the whole set of compounds, the NMDS analysis showed no clear separation between white and purple morphs, but the PERMANOVA revealed a significant colour effect (Table 2). As the interaction term between colour and species effects also proved significant (Table 2), we tested the colour effect independently for each species. These analyses showed that the colour effect revealed by the PERMANOVA (Table 2) was mainly attributable to the significant variation between white and purple morphs of O. simia ( Fig. 2; F(1,16) = 11.38, P < 0.001), whereas for O. mascula and for A. c. fragrans inflorescences the volatile profile showed no significant variation between the two colour morphs ( Fig. 2; O. mascula: F(1,35) = 1.10, P = 0.33; A. c. fragrans: F (1,19) = 0.63, P = 0.73). A few qualitative differences were observed between the two colour morphs of O. mascula. Eleven compounds were found only in the volatile emissions from O. mascula purple inflorescences, while 9 other compounds present in volatiles from white inflorescences were not detected in any of the purple samples. In A. c. fragrans, 4 and 5 compounds were found only in coloured and only in white morphs, respectively. All these “colour-specific” components were found only as traces (<1%), and were found only in a few individuals (fewer than half of individuals for each colour morph). In O. simia, consistent differences were found between the volatile profiles of white- and coloured-flowered morphs. Several compounds showed large differences in their relative proportions between the two colour morphs. The variation was particularly clear for (Z)-3-hexenyl acetate (17% in the white morph, 3% in the coloured morph) and for NI (not identified, 2% in the white morph, 10% in the coloured morph). The NMDS analysis conducted on the most abundant compounds (>1%) showed a separation of the O. simia white and coloured morphs ( Fig 2).
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