Acetobacter require trace nutrients in addition to alcohol. Wine and other fermented fruit juice Vinegar may also be produced through the fermentation of other types of alcohol, either in the form of fermented fruit juice or in the form of pure alcohol. Acetobacter cannot tolerate a high alcohol concentration, and pure alcohol must typically be diluted to about 12 percent for vinegar production. The Kosher status of vinegar produced from pure alcohol is based primarily on the source of the ethyl alcohol.