Supplementation of carbon sources in the form of
monosaccharides, disaccharides and polysaccharides resulted
in marginal increase in a-amylase production by
B. cereus during solid-state fermentation using wheat bran.
Highest production was observed with glucose ((122±5)
U/g) (Fig. 2). Bacillus thermooleovorans is reported to prefer
starch, glucose, lactose, maltose and maltodextrins as carbon sources for a-amylase secretion (16). In contrast,
carbon sources such as glucose, maltose and starch did not
enhance a-amylase production by thermophilic B. coagulans
in solid-state fermentation using wheat bran (11).
Addition of organic nitrogen sources such as casein, yeast
extract and urea, and inorganic nitrogen source such as
ammonium chloride to the medium resulted in considerable
decrease in a-amylase production by B. cereus
(Fig. 3).