In this study, three lactic acid bacteria (LAB), isolated from barley, traditional dried meat and fermented
olive were characterized and tested for their anti-bacterial and anti-biofilm activities against oral
bacteria.
Our results revealed that the tested LAB were g-hemolytic and were susceptible to four antibiotics. All
the strains were resistant to low pH, bile salt, pepsin and pancreatin. Furthermore, FB2 displayed a high
aut-oaggregative phenotype (99.54%) while FF2 exhibited the best co-aggregation rate. Concerning the
microbial adhesion to solvent, FB2 was the most hydrophobic strain (data obtained with chloroform and
n-hexadecane). In addition Pediococcus pentosaceus FB2 and Lactobacillus brevis FF2 displayed a signifi-
cant inhibitory effect against Streptococcus salivarius B468 (MIC ¼ 10%). Moreover the selected strains
were able to inhibit biofilm formation of Bacillus cereus ATCC14579 (MBIC50 ¼ 28.16%) and S. salivarius
B468 (MBIC50 ¼ 42.28%).
The selected LAB could be considered as candidate probiotics for further application in functional food
and mainly in the prevention of oral diseases.