The antioxidant properties of food matrices are due to the
presence of a complex mixture of compounds of varying polarity.
Thus, we decided to use four methods (based on differ mechanism
of action) for determine antioxidant activity of designed products.
In most studies only “chemical” extraction was taken into account.
In the study concerning the influence of extraction conditions on
antioxidant capacity Michiels et al. (2012) proposed standardization
of sample preparation and extraction to allow comparing the
antioxidant capacities and phenolic contents of different food
matrices. Generally, this procedure (similarly to other chemical
extraction) is by all means correct and is useful for fast evaluation
of antioxidant potential, however it does not reflect the conditions
occurring in the gastrointestinal tract/human organism while this
issue is crucial to assess the biological activity of new products.