Many methods have been developed for specifically determining Trp in food samples, such as spectroscopy, high-performance
liquid chromatography (HPLC), fluorometric methods, capillary
electrophoresis and electro analysis (Nurit, Tiessen, Pixley, &
Palacios-Rojas, 2009). Fluorometric techniques have probably
been the most popular due to its high sensitivity, high accuracy,
simple operation mode and low cost
liquid chromatography