The oyster mushroom grown on substrates R80 + WB20 and WS80 + WB20 had significantly lower mushroom weight than that on substrate C80 + WB20. The oyster mushroom grown on substrates C45 + R45 + WB10 and C30 + R60 + WB10 gave significantly higher mushroom weight than that on substrate R80 + WB20 for sterilized substrate, and on substrate C45 + R45 + WB10 gave significantly higher mushroom weight than that on substrate R80 + WB20 for non-sterilized substrate. Oyster mushroom grown on substrates C40 + WS40 + WB20 and C30 + WS50 + WB20 gave significantly higher mushroom weight than that on WS80 + WB20 for sterilized substrate, and substrate C40 + WS40 + WB20 gave significantly higher mushroom weight than that on substrate WS80 + WB20 for non-sterilized substrate. When compared to sterilized substrate, the mushroom on non-sterilized sterilized substrates C45 + R45 + WB10, C30 + R60 + WB10, C40 + WS40 + WB20, C30 + WS50 + WB20 and C80 + WB20 has significantly larger mushroom weight.