To test if those fatty
acids which had a positive effect on sperm viability prolonged also the sperm motility, the
following experiment was conducted. The described fatty acids were
dissolved in buffered sperm motility-activating and prolonging saline
solution
Five-hundred μl saline solution was
pipetted in a 1.5 ml Eppendorf tube and 0.5–1 μl semen was added
and vigorously mixed. After 0, 60, 120, and 180 s, subsamples of 100 μl
were pipetted into the Makler investigation chamber and motility was
recorded. To evaluate the fertility 10 g of eggs were transferred into
petri dishes and 5 ml of a 4 °C cold fertilization solution