Fresh leaves of A. vasica and Lantana camera and bulbs of Allium
satium were harvested and thoroughly washed in tap water 3 to 4
times. 1 00 g of leaves or bulbs of each were macerated to paste
with the help of sterilized mortar and pestle with 1 00 ml of sterilized
distilled water and it was filtered through the muslin cloth. The
extract was then centrifuged at 5,000 rpm for 1 5 min. To obtain
solvent extracts, 1 00 g of finely powdered dry samples were
homogenized in 1 00 ml solvent (methanol) (1 /1 w/v). The mixture
was kept in room temperature (26 ± 2°C) for 4 h, then it was
centrifuged at 5,000 rpm for 1 0 min. Subsequently, the supernatant
was evaporated completely and the residue was dissolved in sterile