Preparation of fermentation media and starter
Fermentation media used for this research is molasses. Molasses was diluted to 24oBrix and the pH set to 4,3
using citric acid or NaOH 0,4 M. Furthermore, molasses which has already prepared was settled for 24 hours to
stabillize the pH and insoluble solids in molasses then removed by filtration. The nutrition for molasses as
fermentation media enriched by adding 100 ppm of Diamonium Hidrogen Phospat (NH4)2HPO4. The prepared
media was sterilized at 121o C for 15 minutes. Starter from commercial yeast was made by weighing the yeast for
1% (w/v) from the total media used in fermentation, then mixed it with glucose solution 2% ± at temperature 42oC
and settled for 3 hours in laminar air flow