Tone of the amplification products generated
by either RAPD or ISSR or SSR primers was
found to be common to all the species. Most of
the RAPD as well as the SSR primers produced
fingerprints that were diagnostic for each
species. On the basis of this analysis, a few
RAPD, ISSR and SSR primers were recognized
(Table 3) that could preferably be used to
fingerprint Cassia germplasm resources. To
characterize the capacity of each RAPD, ISSR
and SSR marker to reveal polymorphic loci in
Cassia, we analyzed the PIC content. In
calculating the PIC, the markers with band
frequencies below 0.05 or above 0.95 were not
considered, because this level is estimated to
be too close to the empirical threshold for the
detection of differences by RAPD and SSR
analyses (24). The calculated PIC values for the
RAPD, ISSR and SSR markers ranged from 0.08
to 0.45. RPI, IPI and SPI values ranged from 0.35
to 4.14, 0.45 to 2.25 and from 0.45 to 2.10
respectively (Table 3).