Taking into
account the results obtained previously, the optimal concentration of
inulinases for winter juice (0.25 mL L−1) was added before the heat
treatment in all cases. The highest ethanol yield (0.454 ± 0.010 g g−1;
88.8% of the theoretical maximum value) was obtained at 52.5 °C in
around 70 h, which was slightly higher than the ethanol yield reached
with T3 (0.446±0.012 g g−1), but considerably higher than the ethanol
yield obtained with the heat treatment T1 (0.372 ± 0.015 g g−1).