Calibration of pH probe was done
before sterilizing the medium as per manufacturer’s in struction.
Dissolved oxygen (DO) was calibrated after
polarizing or before adding inoculums into the bioreactor
as per manufacturer’s manual instruction. The initial batch
fermentation condition of bioreactor was as follows: culture
medium volume, 3.7 L; 5% inoculum, temperature,
37C; airow rate, 3 LPM; and agitation speed, 200 rpm. A
nal concentration of 30 mg/mL of kanamycin was added
into the fermentation vessel by injection through injectable
port.