Purified recombinant gGILT was added to 0.1% TritonX-100 in 100 mM NaCl, 50 mM acetate, pH 4.5, to afinal volume of 100ml, and gGILT was preactivated with 25mM freshly prepared DTT at 37 C for 10 min.
Purified recombinant gGILT was added to 0.1% TritonX-100 in 100 mM NaCl, 50 mM acetate, pH 4.5, to afinal volume of 100ml, and gGILT was preactivated with 25mM freshly prepared DTT at 37 C for 10 min.