Excellent lactic acid bacteria strains and efficient and compound starter cultures were screened which are suitable for the fermentation of marigold flowers. The effect of lactic acid bacteria inoculation quantity, fermentation temperature and time on the fermentation of marigold flowers was determined in the experiment. The determined optimum technical parameters for marigold flowers fermentation is as follows: inoculated quantity flowers; 0.2% of heavy fermentation temperature is 20 to 40 ℃; fermentation time for 15 to 20 d. The fermentation result shows that the fermented marigold looks gorgeous and colorful, there is no decay or rot occurred, and the fermentation liquid is clean and clear, which indicates that the fermentation effect is desirable and ideal compared with the current processing method used in most enterprises.