Propionibacterium acne was incubated in medium with 1% glucose for 72 hour under anaerobic condition and adjusted to yield approximately 1.0×10 cfu/ml. Aliquots of molten BHI with glucose agar were used as an agar base.A prepared inoculum was added to molten agar,mixed and poured over the surface of the agar base and left to solidity.A sterile paper disc was impregnated with test material and the disc was placed on the agar.Plates were then incubated at 37 c for 72 hour under anaerobic conditions.