Up to 50% of endocytosed fluid-phase markers can also be rapidly
recycled from endocytic compartments [1], therefore we
examined if PLA2 antagonists also inhibit recycling of a fluid-phase
marker, fluorescent Lucifer yellow (LY). When cells were pulse-labeled
by the endocytic uptake of LY and chased for various periods
of time, ONO slowed the loss of LY from cells (Supplemental
Fig. 1A). LY recycling was presumably slowed because the membrane
tubules mediating recycling of TfR and LDLR also carry significant
amounts of fluid-phase.