ree polyamine endogenous levels was measured and
showed higher contents for the TIS-TF, followed by TIS-RITA®, and PIS, respectively. Putrescine levels also
followed this pattern, being absent in PIS, which, on the other hand, showed higher levels of spermine and
spermidine. The results indicate the establishment of efficient protocol for pineapple micropropagation,
with a high multiplication and regenerative rates and great potential for agriculture application. The
biochemical analysis suggests that besides culture medium composition, flask design and culture system
also drastically affects the plant’s metabolism toward multiplication and plant regeneration.