the leaves of chrysanthemum were selected at 0,15,30,45,60 Das for measurement.
soluble sugar and soluble protein measurement were followed the procedures that were described by Frohlish and Kurscherah 1990.
soluble sugar was extracted with anthrone.
samples(0.30g) of fresh leaves were put into test tubes with 10 ml. distilled water and sealed.
the tube were incubated in a water bath at 90 c for 30 min, then the tube were removed and the volume set 25 ml. 0.5 ml anthrone and 5 ml concentrated sulfuric acid.
the mixed solution was read at 620 nm for soluble sugars measurement.