The total amount of collected samples was less than 5% respect to the total volume. Samples are filtered, cooled, diluted in 1000 μl of acetone and analysed by HPLC. Three mass ratios biocatalyst/oil (E0/Oil0) were investigated: 1%, 3% and 5% w/w. The substrate molar ratio oil/ethanol (EtOH0/Oil0) was 3:1, feed of ethanol was carried out step by step with feed molar ratio EtOH0/Oil0 for step 1:1 in anhydrous conditions.