Batch cultivation experiments were carried out using phenol as single limiting
substrate for mixed and pure culture. Loop full of biomass is transferred into reactor
with 100mg/l and 200mg/l phenol. The extent of phenol degradation using these
different initial phenol concentrations was investigated for several batch residence
times by intermittent sampling at every six hour interval. The concentration of the
phenol was determined using the colorimetric 4-aminoantipyerene method. [11, 12,
16] using UV-Vis spectrophotometer at 500 nm Wave length.