specificity (a-amylase, b-glucanase, pentosanase, cellulase, protease).
In separate hydrolysis and fermentation experiment following
enzymes were used (all produced by Novozymes): Termamyl SC DS
(thermostable a-amylase from Bacillus licheniformis with declared
activity of 240 kilo novo units per gram) and SAN Extra L (glucoamylase
from Aspergillus niger with declared activity of 400 glucoamylase
units per gram) and, mentioned above, Neutrase 0.8 L.
Commercial active dry yeast Saccharomyces cerevisiae Ethanol
Red (Fermentis, France) was used as production inoculum, it contained
over 20 109 of living cells per gram as declared by the
manufacturer. Prior to inoculation the yeast were rehydrated in
sterile distilled water (1 g of yeast preparation in 10 mL of water)
at 30 C for 0.5 h with regular agitation.