To assay for bacterial invasion, the RCEs were lysed in Triton X-100 (0.25% [vol/vol]; LabChem Inc., Pittsburgh, PA) in PBS for 15 minutes. The cells were scraped vigorously to ensure complete lysis.
To assay for bacterial invasion, the RCEs were lysed in Triton X-100 (0.25% [vol/vol]; LabChem Inc., Pittsburgh, PA) in PBS for 15 minutes. The cells were scraped vigorously to ensure complete lysis.