This experiment aims to do Protein purification Protein visualization Western, and blot analysis to produce proteins VP35. A protein important white-spot virus in infected shrimp living in and make the protein produced is used in the future after the Transformation gene VP35 into cell, will bring cell to do Protein purification to extract to remove protein out by will, Supernatant. Pellet Bead and the Suspension both 3 parts do Protein visualization SDS-PAGE way to see if there is a protein. VP35 or not by the Rf compared with Proteinmarker found that the protein VP35 in suspension of Bead Supernatant then the gel and the from the analysis. SDS-PAGE doing Western blot analysis to see the expression of protein and visualize protein VP35 more clearly see ว่าเกิด strips of protein. VP35 that 35 kDa by the Proteinmarker.