Plants of D. aggregatum
were collected from their natural habitat and kept
under shade net (75%) house environment at the
campus of the Government Arts College, Coimbatore.
After flowering, several flowers were hand pollinated
on the second of anthesis. The pollinated flowers
were bagged with butter paper for one week. Several
capsules of D. aggregatum were harvested 120 days
after pollination and brought to the laboratory for in
vitro seed germination.