pproximately 3 kb of sequence 5′ upstream from the translational start site of gfap and the first 16 bp of Exon1 and ∼4.3 kb of Intron1, were cloned by PCR from a zebrafish BAC containing the gfap gene. These genomic sequences were cloned into a promoterless GFP vector to generate a fusion protein ( Fig. 1).