Soy sauce has been made for centuries by traditional methods, and consumed as the source of protein and vitamins.
In this research work, the two fermenting microbes Aspergillus oryzae and Aspergillus flavus were mainly employed
to produce soya sauce. Aspergillus oryzae was isolated from moldy soybeans. Also, Aspergillus flavus strain was
received from the Department of Biotechnology, Yangon Technological University, Myanmar and recharacterized to
confirm the correct strain. Four types of soy sauce products obtained from separated fermentation of each strain
within two periods of different fermentation time (1.5 months and 3 months) and were analyzed for protein content,
fat content, reducing sugar content, and alcohol content respectively after fermentation. Analysis of commercial soy
sauce product from local market was also done as a comparative study for locally strain produced products. Finally,
amino acid composition of each product was detected by the Thin Layer Chromatographic method as a part of
research work. By comparing the physical and chemical analysis results, soy sauce produced by A. oryzae, brine
ageing time 3 months had the best quality.
Soy sauce has been made for centuries by traditional methods, and consumed as the source of protein and vitamins.
In this research work, the two fermenting microbes Aspergillus oryzae and Aspergillus flavus were mainly employed
to produce soya sauce. Aspergillus oryzae was isolated from moldy soybeans. Also, Aspergillus flavus strain was
received from the Department of Biotechnology, Yangon Technological University, Myanmar and recharacterized to
confirm the correct strain. Four types of soy sauce products obtained from separated fermentation of each strain
within two periods of different fermentation time (1.5 months and 3 months) and were analyzed for protein content,
fat content, reducing sugar content, and alcohol content respectively after fermentation. Analysis of commercial soy
sauce product from local market was also done as a comparative study for locally strain produced products. Finally,
amino acid composition of each product was detected by the Thin Layer Chromatographic method as a part of
research work. By comparing the physical and chemical analysis results, soy sauce produced by A. oryzae, brine
ageing time 3 months had the best quality.
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