The effect of direct-diffusible metabolites from T. viride, T.
polysporum, T. hamatum, and T. aureoviride against C. paradoxa
were determined by transferring seven days old mycelial plug (4
mm diameter) removed from growing edge each of Trichoderma
species to the center of Petri dishes containing PDA, previously left
overnight to allow excess water to evaporate. The uninoculated
Petri dishes were placed over the inoculated Petri dishes of
Trichoderma species and attached firmly by tape. The Petri dishes
were untapped after 3 days. Seven old mycelial disc (4 mm
diameter) of C. paradoxa was inoculated on previously
uninoculated Petri dishes. The control sets did not contain the
antagonist, only had the uninoculated Petri dishes placed over the
same uninoculated Petri dishes and attached also firmly by tape.