The bene fi t of disacchar ide protectants for maintain ing via bility and b -gluco sidase activity of Bifi do-bacteriu m infantis UV16PR during freeze-drying and storage in different food ma trices was investig ated.
Protectants used were cell obiose, lactose, sucrose and t rehalose.
At 5% conc entration, cell obiose showed the be st protective effect during freeze-drying. All protectan ts
at 10% conc entration signi fi cantly improved t he via bility and retenti on of b -gluc osidas e activity af ter
freeze-drying and storage in food matrice s. Reg ardless of the protectant used, no sign ifi cant dif ferences
were obs erved af ter freeze-drying and storage for 2 weeks in milk , while in red-beet and grape ju ice,
cello biose and trehalose signi fi cantly enhanc ed b-gluc osi dase activity, via bility and acid tolerance .
Viability of cello biose and t rehalose protected cells in juic es was comparable, wh ereas differences in
retenti on of enzyme activity and acid tolerance af ter the storage in different juic es were obs erved. In
conclus ion, vari ous protectants in creased the retenti on of b -gluc osidase activity, viability and acid
tolerance of freeze-drie d B. infantis dur ing storage in different food ma trices. In order to choos e pro-tectants for probiotics for freeze-drying and food as probiotic ca rrier, not only their effec t on surviv-ability, but also t he enzyme activity retention should be conside red
The bene fi t of disacchar ide protectants for maintain ing via bility and b -gluco sidase activity of Bifi do-bacteriu m infantis UV16PR during freeze-drying and storage in different food ma trices was investig ated.
Protectants used were cell obiose, lactose, sucrose and t rehalose.
At 5% conc entration, cell obiose showed the be st protective effect during freeze-drying. All protectan ts
at 10% conc entration signi fi cantly improved t he via bility and retenti on of b -gluc osidas e activity af ter
freeze-drying and storage in food matrice s. Reg ardless of the protectant used, no sign ifi cant dif ferences
were obs erved af ter freeze-drying and storage for 2 weeks in milk , while in red-beet and grape ju ice,
cello biose and trehalose signi fi cantly enhanc ed b-gluc osi dase activity, via bility and acid tolerance .
Viability of cello biose and t rehalose protected cells in juic es was comparable, wh ereas differences in
retenti on of enzyme activity and acid tolerance af ter the storage in different juic es were obs erved. In
conclus ion, vari ous protectants in creased the retenti on of b -gluc osidase activity, viability and acid
tolerance of freeze-drie d B. infantis dur ing storage in different food ma trices. In order to choos e pro-tectants for probiotics for freeze-drying and food as probiotic ca rrier, not only their effec t on surviv-ability, but also t he enzyme activity retention should be conside red
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