Pre (0 to 1 h)- and post (24 h)-CR feeding venous blood samples collected from all calves were refrigerated
for a minimum of 6 h and allowed to clot. They were then centrifuged (model DSC-158T Digisystem Laboratory Instruments Centrifuge; Digisystem Laboratory Instruments Inc., New Taipei City, Taiwan) at 10,000 rpm for 10 min and serum was separated.