The prepared crude extract was divided into two portions; one
portion (10 g) was poured into the glass vials to be tested as crude
methanol extract for antimicrobial activity, while the second portion
(100 g) was taken in a glass beaker for fractionation with different
solvents. The second portion was dissolved in water, poured into a
separatory funnel and then distilled petroleum ether was added into
it. The separatory funnel was shaken to separate the two phases as
petroleum ether being immiscible with water