Developed in 1983 by Kary Mullis,[2][3] PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications.[4][5]These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (used in forensic sciences andDNA paternity testing); and the detection and diagnosis of infectious diseases