low nutrient and lipid are problem digestion of cattle, recombinant S. cerevisiae harboring pfaE gene from S. putrefaciens ้้has similar propertieis yeast are source of lipid and can digestion of fiber. When recombinant S. cerevisiae harboring pfaE gene from S. putrefaciens supplement with roughage (low nutrient) responded to increase digestibility in rumen of Thai native cattle.