2.4. Elongation and root development
After 10 months of culture, seedlings, about 0.5 cm long, obtained
from in vitro germination were inoculated into flasks (6.2 cm diameter
and 12.5 cm length) containing 40 ml of WPM medium supplemented
with 1, 2 and 3 g L−1 activated charcoal. Six seedlings were cultured
in each flask with 10 replicates, for a total of 60 seedlings per treatment.
After 16 weeks, the average number of roots, the average length of the
three largest roots and the average length of the shoot were recorded.