Preparation of chitosan nanoparticle containing vitamin C
A chitosan solution was prepared by dissolving chitosan in 1% (w/v) acetic acid solution until the solution was transparent. Sodium tripolyphosphate (0.5 mg/ml) was dissolved in deionized water.
The chitosan solution was flush mixed with an equal volume of TPP solution and the formation of chitosan–TPP nanoparticles began spontaneously via the TPP ionic gelation mechanism.