Platelet concentrate should be prepared by centrifugation of a single
unit of whole blood collected with a smooth venepuncture and a
continuous flow of blood. Platelet concentrate should be separated from whole blood within 8
hours of collection by centrifugation at 220C + 20C using either platelet
rich plasma (PRP) or buffy coat (BC) method, which is validated.
Platelet concentrate prepared from whole blood (450 ml) should contain
a minimum 4.5x1010 platelets and from 350 ml whole blood minimum
of 3.5x1010 platelets in at least 75% of units tested on storage from 1%