Summary of points to include in the discussion:
1. The answers to the experimental questions
2. Supporting material for the answers (from experimental results and reference to supporting articles when required)
3. An explanation of why the answer is reasonable (with references to supporting articles when required)
4. An explanation of results that do not support the answer
5. An explanation of why any of the results are different to published results
6. An explanation of any unexpected results
7. A statement and explanation of any limitations or weaknesses in the method or study design
8. Statements regarding the importance of the answer (outcome) and the impact of the new knowledge (what happens next?)