Simultaneous saccharification and fermentation (SSF) is fementation ethanol from lignocellulosic raw materials by hydrolysis the fermentation.The Saccharomyces cerevisiae strains used in this Of the 50 strains of S. cerevisiae isolated from Brazilian alcohol only strains S.cerevisiae LBM-1, which can grow best at temperatures of 42° C Selected to study ethanol production ready to Kluyveromyces marxianus UFV-3 K. marxianus strain that showed fermentation results when converting lactose in cheese whey to ethanol. shows that all tested dilutions of the two strains grew at temperatures of 30, 37 and 42 ° C found that Kluyveromyces marxianus UFV-3 can grow better than other varieties. Mill-dried in natura sugarcane bagasse was pretreated with diluted sulfuric acid and resulted when comparing the SSF process with presaccharification to the SSF process without presaccharification. fermentation in this study by using a K. marxianus strain that ferments at 42° C Found in the SSF presaccharitication with 72-hour time step at the temperature 50 ° C and 8 hours of marinating, another ethanol output maximum lasung 1.79 grams per liter. That is higher than the traditional fermentation process SSF that no process of presaccharitication hydrolysis and fermentation, and Separate the fermentation (SHF), which is presaccharitication, the output is less than the fermentation process of SSF with presaccharitication as well.