Somatic hybridization may be a useful tool for the genetic modification of chemical composition
of the hybrid plant. This study was designed to improve the secondary metabolite by somatic
hybridization of medicinally important plants Murraya koenigii and Centella asiatica. The
protoplasts were isolated by enzymatic method and they were fused with PEG. The hybrid
protoplasts were selected using drug sensitivity method (Tetracycline). For establishing the
microcalli B5 medium enriched with BA 1.00 mg/l,BA 2.00 mg/l showed higher activity. The
combination of BA and NAA or IAA at different levels induced callus. The methanolic extracts
of hybridized callus and parental plants were prepared. Secondary metabolites produced were
seperated by TLC and compared by their obtained Rf value and antibacterial activity. The work
showed fused callus extracts with higher Rf value and also showed more antibacterial activity
than the parental extracts, however the study is a stepping stone for improvement of secondary
metabolite