Under the optimized SFE conditions, SFE extraction was scaled up 30-fold using a 1000ml vessel. A 195g amount of sample was extracted statically for 1h, and after dynamic extraction for 3 h by flowing liquid CO2 at a rate of 2lmin−1, the extract was depressed directly into a collection vessel and stored in a refrigerator for sub-sequent HPLC analysis and HSCCC separation