A typical method of
production of the vesicles is by sonication of
solution as described by Cable (32). In this
method an aliquot of drug solution in buffer is
added to the surfactant/choleste mixture in a
10-ml glass vial. The mixture is probe
sonicated at 60°C for 3 minutes using a
sonicator with a titanium probe to yield
niosomes.( Fig 5).