the reduced zeta potential of the released AgNP indicates
surface modification by the NRL molecules, which
stabilize the AgNP from a steric viewpoint. FTIR
spectra suggest that AgNP drag non-rubber molecules
from NRL. These non-rubber molecules attached to
the AgNP are associated with the NRL serum fraction
and may be responsible for steric stabilization. The
AgNP attached to the cis-isoprene molecules in the
NRL matrix remain attached to the membrane. So,
only the AgNP bound to the non-rubber molecules are
released. Because the NRL serum fraction is responsible
for the angiogenic properties of NRL, the silver
nanoparticles could potentialize the angiogenic properties
intrinsic to the NRL by increasing the release of
the molecules responsible for angiogenesis. These
results reveal that this biohybrid nanomaterial features
properties for fabrication of a wound dressing with
potential healing action. This material combines the
angiogenic characteristic of NRL with the antibacterial
properties of the AgNP and AgNP potentialize the
NRL angiogenic activity. Future studies will focus on
the influence nanoparticle size on the AgNP release
rate, as well as on the tissue reaction after implant and
the performance of the biohybrid material as wound
dressing.