The medium was centrifuged at 10000g for 15 min at 4 °C.
The pellet following centrifugation was washed with 30 mL
of methanol and centrifuged, and the resulting supernatant
was combined with the initial extract. Triplicate supernatant
extractions were made for each sample. The methanolic extract
volume was reduced in the evaporator to 20 mL and then made
up to 50 mL with methanol.