specific primers were designed targeting at the gene coding for the VP6 protein of the RVs-D, using the Primer BLAST program available at NCBI. The forward primer used in this study was RD6F (5-GGAGGCGCTGTCTTCAATTGCG-3) and the reverse primer was RD6R
(5-TGGCCAATAGTGTGTGGCAGCT-3), which were used to amplify a 742-bp fragment.