Fifteen hundred grams of healthy cassava stem pieces was thoroughly washed in running tap water.
The periderm and sclerenchyma tissues were peeled off with a knife and the central stem tissues (cortex) were dried at 60° C to constant weight, for 72 h.
The dried pieces were chopped ,
and crushed into powder using a warring blender (Cookworks Mod el BL907 2; Cookworks, Dallas, Texas).
Three hundred grams of powder were added to1000 ml of sterile distilled water,
and allowed to shake on an electric shaker for 10 min.
The extract was filtered through a three-layered muslin cloth,
and the filtrates collected in conical flask then stored at 10 °C in an incubator for immediate use