Evaluation of physicochemical parameters
Physicochemical parameters of bile such as color, pH, specific gravity, Rf value were determined along with stability study. The colour observed by naked eyes, pH was measured by using digital pH meter (Eutech Instruments pH tutor Singapore) and specific gravity was determined by adopting a standard method [10]. Rf value of crude bile content was determined by TLC using silica gel G as stationary phase, various solvent and combinations as mobile phase, iodine vapors used for spot detection [11]. Further qualitative chemical tests for the presence of various organic and inorganic substances were also carried out by adopting the standard procedures [10, 12, 13].