Two plasmids, a vector plasmid containing the transposable element into which a gene of interest and a genetic marker have been cloned, and a helper plasmid containing the relevant transposase gene, are microinjected into blastoderm embryos
Two plasmids, a vector plasmid containing the transposable element into which a gene of interest and a genetic marker have been cloned, and a helper plasmid containing the relevant transposase gene, are microinjected into blastoderm embryos