Aphelenchoides besseyi was isolated from infected seeds collected from
rice fields in Guilan province, north of Iran. Nematodes were extracted
according to Coolen and D'Herde's method (Coolen and D'Herde, 1972). A.
besseyi can be cultured on various fungi. In this study, the nematode multiplied
on Alternaria alternata cultured on PDA (potato dextrose agar) because the
highest reproduction was achieved on it (Jamali et al., 2008).