The cultivated A. aceti cells were collected by
centrifugation for 10 min at 2900 * g and then washed with distilled water twice. A
sample of the collected cells, possessing a wet cell weight of 0.2 g, was suspended in
50 ml of the fermentation medium, which contained 1% (w/v) peptone, 1% meat
extract and 0.5% ethanol. The resulting A. aceti cell suspension in the fermentation
medium, i.e., the fermentation suspension, was injected into the MFC anode
chamber through the hole, and the hole was then sealed to create an anaerobic
environment within the anode chamber